Molecular Applications in Zoology

Molecular Applications in Zoology

Lectures will be every Tuesday at 1.10 PM (13:10) in the room B312 (3rd floor, Viničná 7). The course starts on Tuesday February 25, 2025.

1.  Introduction, DNA extraction, PCR and electrophoresis (lecturer Zuzana Starostová) 

2. How to read and make phylogenetic trees (lecturer Zuzana Starostová)

3. Phylogenetic trees II + use of molecular phylogenetics in zoology (lecturer Zuzana Starostová)

4. Molecular phylogeography (lecturer Pavel Hulva)

5. Next generation sequencing – introduction, short-read and long-read sequencing methods, assemblies (lecturer Radka Reifová)

6. Next generation sequencing – applications: whole genome sequecing, exome sequencing, ddRAD sequencing, metagenomics  (lecturer Radka Reifová)

7. Microsatellites and molecular identification of species, individuals and sex (lecturer Pavel Munclinger)

8. Population structure and paternity studies (lecturer Pavel Munclinger)
    Article about parentage analysis. Links to programs from the lecture: Cervus, PopG

9. Gene expression – quantitative PCR, expression microarrays, transcriptome sequencing and analysis, epigenomics (lecturer Radka Reifová)

10. Functional genetic variability: From SNP to selection (lecturer Michal Vinkler)

11. Cytogenetic methods and their application in zoology (František Šťáhlavský )

Practicals:

presentation - computer part